SSR markers assay confirms genetic fidelity for regeneration of true to type micropropagated plants in crocus sativus L.

Vol-8 | Issue-10 | October-2021 | Published Online: 15 October 2021    PDF ( 295 KB )
DOI: https://doi.org/10.53573/rhimrj.2021.v08i10.002
Author(s)
Kuldeep Raj 1; K.S. Chundawat 2

1PhD scholar, Department of life sciences, Mewar University, Chittorgarh (Rajasthan) India.

2Professor, Department of life sciences, Mewar University, Chittorgarh (Rajasthan) India.

Abstract

In vitro regeneration is very important and useful technique for the large scale multiplication of the many plant species. This technique is also used for the mass multiplication of many recalcitrant plant species. In micropropagated plant clonal fidelity is one of the most important pre-requisites. Different types of the molecular markers have been used for the detection of the clonal fidelity in the in vitro regenerated plants. During present study attempts has been made to develop an effecient, reliable and quick regeneration protocol in croccus sativus using corms as explants. The genetic stability of these plants was checked by SSR molecular markers.

Keywords
Microsatellite, polymorphic, micropropagation, genetic stability
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